Gel Electrophoresis Buffers and Diluents
Research Products International Corp Dry Powder Milk, 1 Kilogram
Nonfat dry milk for use as a blocking agent during Western Blotting to block non-specific antibody binding and reduce background signal.
![Research Products International Corp TGS 10X Solution [TRIS-Glycine-SDS Buffer 10X Solution], 5 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T32080-5000.0.JPG-250.jpg)
Research Products International Corp TGS 10X Solution [TRIS-Glycine-SDS Buffer 10X Solution], 5 Liters
Common buffer used for running SDS-PAGE gels in electrophoresis.
1X Solution Contains:
TRIS/TRIS HCl 25 mM
Glycine 0.192 M
SDS 0.1%(w/v)
New England Biolabs, Inc. Gel Loading Dye Purple (6X) – 4 ml
Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands. It is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes. Attention SYBR Safe and GelRed dye users: Due to an increased concentration of SDS in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.
Seracare Life Sciences Inc BCIP/NBT 1-C AP SUBSTRATE
Effective chromogenic substrate for use in blotting, it provides crisp bands of intense purple precipitate with minimal background staining. Designed for use with alkaline phosphatase-labeled conjugates. Recommended for blotting and ELIspot Assays. 1-Component format of BCIP/NBT (5-bromo-4-chloro-3-indoyl-phosphate/nitroblue tetrazolium), no reagent preparation, hence, more convenient.
Zymo Research Corporation M-Desulphonation Buffer, 10 ml
M-Desulphonation Buffer (10 ml) for use with EZ DNA Methylation™ bisulfite conversion Kits. Sold individually or as part of a bisulfite conversion kit.
Zymo Research Corporation M-Binding Buffer, 80 ml
M-Binding Buffer (80 ml) for use with EZ DNA Methylation™ bisulfite conversion Kits.Sold individually or as part of a bisulfite conversion kit.
Zymo Research Corporation ZymoPURE™ Binding Buffer (110 ml)
ZymoPURE Binding Buffer is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Zymo Research Corporation Buffer P2 (Green), 20ml
Buffer P2 (Green) is designed to be used with our ZR Plasmid Miniprep - Classic kit, which is used to efficiently isolate ultra-pure plasmid DNA from E. coli using Zymo-Spin column purification technology.
Research Products International Corp Acrylamide, 500 G
Acrylamide (C₃H₅NO) is a water-soluble monomer (71.08 g/mol) that forms polyacrylamide gels used in electrophoresis to separate proteins and nucleic acids. It polymerizes with bis-acrylamide and initiators like APS and TEMED. Common applications in life science labs include SDS-PAGE, Western blotting, and nucleic acid analysis.
WARNING! This product can expose you to Acrylamide, a chemical which is known to the State of California to cause cancer, developmental and reproductive harm. For more information go to www.P65Warnings.ca.gov.
Revvity Health Sciences Inc HTRF Reconstitution Buffer #2, 50 mL
HTRF Reconstitution Buffer #2, 50 mL
Zymo Research Corporation RNA Lysis Buffer, 50 ml
RNA Lysis Buffer (50 ml). For use with a wide variety of RNA purification kits from Zymo Research. Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
Boca Scientific Inc LowCross-Buffer, 50ml Minimize nonspecific binding, cross-reactivities and matrix effects in immunoassays.
LowCross-Buffer is an innovative assay buffer for ELISA, EIA, RIA, protein arrays, Western blotting, immunohistochemistry and immuno-PCR. It is ready-to-use and can replace your standard sample or antibody dilution buffer. The formulation of LowCross-Buffer helps to reduce immunoassay interference and can therefore greatly improve the quality of detection. Nonspecific binding of the antibodies, negative effects of interfering substances, and low or medium affinity cross reactivities of the antibodies can be significantly decreased. Matrix effects, such as those from blood sera or plasma specimens, are minimized. Reduction of these negative effects improves the quality of the assay and the reliability of the results.